NGEF antibody (pTyr179)

Catalog No. ABIN1713981

The Rabbit Polyclonal anti-NGEF antibody (ABIN1713981) specifically detects NGEF in WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NGEF antibody (pTyr179) (ABIN1713981)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGEF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-NGEF Antibody

Binding Specificity: pTyr179

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr179

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: Ephexin-1

Background:

Synonyms: Ephexin-1 phospho Y179, p-Ephexin-1 phospho Y179, ARHGEF27, Eph-interacting exchange protein, EPHEXIN, Ephexin 1, Neuronal guanine nucleotide exchange factor, Ngef, NGEF_HUMAN.

Background: EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.

Gene ID: 25791

Pathways: Neurotrophin Signaling Pathway