ARF1 antibody (AA 35-130)

Catalog No. ABIN1713983

The Rabbit Polyclonal anti-ARF1 antibody has been validated for ELISA, IF (cc), ICC, IF (p), IHC (p) and IHC (fro). It is suitable to detect ARF1 in samples from Rat.

Quick Overview for ARF1 antibody (AA 35-130) (ABIN1713983)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ARF1 Antibody

Binding Specificity: AA 35-130

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ARF1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background:

Synonyms: ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1, ARF1_HUMAN, DJ133P16.1.

Background: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

Gene ID: 375

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process