Cytohesin 2 antibody (AA 21-120)

Catalog No. ABIN1714087

Product Details anti-Cytohesin 2 Antibody

Target: Cytohesin 2 (CYTH2)

Binding Specificity: AA 21-120

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytohesin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cytohesin 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cytohesin 2

Target: Cytohesin 2 (CYTH2)

Alternative Name: Cytohesin 2 (CYTH2 Products)

Synonyms: ARNO antibody, CTS18 antibody, CTS18.1 antibody, PSCD2 antibody, PSCD2L antibody, SEC7L antibody, Sec7p-L antibody, Sec7p-like antibody, CLM2 antibody, Pscd2 antibody, Arno antibody, Sec7 antibody, Sec7B antibody, pscd2 antibody, MGC84366 antibody, CYTH2 antibody, cyth2 antibody, cytohesin 2 antibody, cytohesin-2 antibody, cytohesin 2 L homeolog antibody, si:dkey-283b15.4 antibody, CYTH2 antibody, PTRG_08834 antibody, Cyth2 antibody, cyth2.L antibody, cyth2 antibody, si:dkey-283b15.4 antibody

Background:

Synonyms: ARF exchange factor, ARF nucleotide binding site opener, Arno, ARNO protein, CLM2, CTS18, CTS18.1, Cyth2, Cytohesin 2, MGC137537, MGC80440, PH, SEC7 and coiled-coil domain-containing protein 2, Pleckstrin homology Sec7 and coiled coil domains 2, Pleckstrin homology Sec7 and coiled coil domains protein 2, PSCD2, PSCD2L, PSCD2L, formerly, Sec7, SEC7 homolog B, Sec7B, SEC7L, Sec7p L, Sec7p-like, Sec7pL, CYH2_HUMAN.

Background: The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf?s activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).

Gene ID: 9266