NGFR antibody (AA 151-250)

Catalog No. ABIN1714472

The Rabbit Polyclonal anti-NGFR antibody has been validated for WB, FACS, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It is suitable to detect NGFR in samples from Rat and Mouse.

Quick Overview for NGFR antibody (AA 151-250) (ABIN1714472)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NGFR Antibody

Binding Specificity: AA 151-250

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NGFR/p75NTR

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background:

Synonyms: Gp80 LNGFR, ngfrp75, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, NGF receptor, p75 ICD, CD271, CD271 antigen, Gp80 LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor, Nerve growth factor receptor TNFR superfamily member 16, NGF receptor, NGFR, p75 ICD, p75 Neurotrophin receptor, p75 NTR, TNFR Superfamily Member 16, TNFRSF16, Tumor necrosis factor receptor superfamily member 16, TNR16_HUMAN.

Background: The low affinity NGFR (Nerve growth factor receptor) is a 75 kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis.

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding