TBP antibody (AA 1-20)

Catalog No. ABIN1722676

This Mouse Monoclonal antibody specifically detects TBP in WB, IHC, ELISA, IP, ICC, IF, FACS, EMSA and ChIP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TBP antibody (AA 1-20) (ABIN1722676)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Electrophoretic Mobility-Shift Assay (EMSA), Chromatin Immunoprecipitation (ChIP)

Clone: 1TBP18

Product Details anti-TBP Antibody

Binding Specificity: AA 1-20

Specificity: Reacts with human, mouse and rat TATA Binding Protein. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus.

Purification: Purified (protein G)

Immunogen: Recombinant human TBP (epitope within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.)

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(2 μg/mL- ELISA (1:100 - 1:2000) Gel Super Shift Assays (1:1 - 1:100) IP(1:10 - 1:500) ChIP(5 - 10 μg for 25 μg of chromatin.)

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C/-80 °C

Storage Comment: Stable for at least one year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TATA Binding Protein

Background: TATA-box-binding protein TATA sequence-binding protein TATA-binding factor TATA-box factor Transcription initiation factor TFIID TBP subunit Gene name : TBP, GTF2D1, TF2D, TFIID

Gene ID: 6908

UniProt: P20226

Pathways: WNT Signaling