EGFR Mutant (AA 693-893) antibody

Catalog No. ABIN1724825

The Mouse Monoclonal anti- antibody has been validated for ELISA, IHC, FACS and WB. It is suitable to detect in samples from Human. There are 2+ publications available.

Quick Overview for EGFR Mutant (AA 693-893) antibody (ABIN1724825)

Target: EGFR Mutant

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Clone: 5G9B5

Product Details

Binding Specificity: AA 693-893

Purpose: EGFR mutant Antibody

Characteristics: Mutant

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References

Derer, Bauer, Lohse, Scheel, Berger, Kellner, Peipp, Valerius: "Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 11, pp. 5230-9, (2012) (PubMed).

Minder, Bayha, Becker-Pauly, Sterchi: "Meprin? transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35201-11, (2012) (PubMed).

Target Details

Target: EGFR Mutant

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Molecular Weight: 175 kDa

Gene ID: 1956

UniProt: P00533