CD25 antibody (AA 34-139)

Catalog No. ABIN1724861

The Mouse Monoclonal anti-CD25 antibody has been validated for WB, IHC, ELISA and ICC. It is suitable to detect CD25 in samples from Human, Mouse, Rat and Monkey. There are 2+ publications available.

Quick Overview for CD25 antibody (AA 34-139) (ABIN1724861)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 1B5D12

Product Details anti-CD25 Antibody

Binding Specificity: AA 34-139

Purpose: IL2RA Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CD25 antibody (ABIN1724861)

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, (2012) (PubMed).

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, (2012) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA

Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Molecular Weight: 30.8 kDa

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation