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CD14 antibody (AA 20-214)

Name: CD14 antibody (AA 20-214)
Brand: antibodies-online
SKU: ABIN1724926
Price: 533.5 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects CD14 in FACS, WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red). (CD14 antibody (AA 20-214))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD14 antibody (AA 20-214))

ELISA

Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264. (CD14 antibody (AA 20-214))

Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (CD14 antibody (AA 20-214))

Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate. (CD14 antibody (AA 20-214))

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining. (CD14 antibody (AA 20-214))

IHC

6 images

(2 references)

Catalog No. ABIN1724926

Datasheet as PDF

Quick Overview for CD14 antibody (AA 20-214) (ABIN1724926)

Target

See all CD14 Antibodies

CD14

Reactivity

Human, Mouse

Host

Mouse

Clonality

Monoclonal

Conjugate

This CD14 antibody is un-conjugated

Application

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone

4B4F12

Discover our top product CD14 Primary Antibody

Binding Specificity
59

12

8

5

5

4

4

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-214

Purpose

CD14 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.

Isotype

IgG1
anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 Reactivity: Human FACS, WB, IF, IHC (p) Host: Rabbit Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 Biotin

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Matthews, Chung, Klasse, Moore, Sanders: "Potent induction of antibody-secreting B cells by human dermal-derived CD14+ dendritic cells triggered by dual TLR ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 12, pp. 5729-44, (2012) (PubMed).

Haghparast, Heidari Kharaji, Malvandi: "Down-regulation of CD14 transcripts in human glioblastoma cell line U87 MG." in: Iranian journal of immunology : IJI, Vol. 8, Issue 2, pp. 111-9, (2011) (PubMed).
Target

CD14

Alternative Name

CD14

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

Molecular Weight

40 kDa

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades