Nibrin antibody (AA 467-615)

Catalog No. ABIN1724946

Cited in 2+ publications. The Mouse Monoclonal anti-Nibrin antibody (Clone 7E4A2) (ABIN1724946) specifically detects Nibrin in WB, ELISA, IHC, ICC and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for Nibrin antibody (AA 467-615) (ABIN1724946)

Target: Nibrin (NBN)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7E4A2

Product Details anti-Nibrin Antibody

Binding Specificity: AA 467-615

Purpose: NBN Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human NBN (AA: 467-615) expressed in E. Coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Nibrin antibody (ABIN1724946)

Zuhlke, Johnson, Okoth, Stoffel, Robbins, Tembe, Salinas, Zheng, Xu, Carpten, Lange, Isaacs, Cooney: "Identification of a novel NBN truncating mutation in a family with hereditary prostate cancer." in: Familial cancer, Vol. 11, Issue 4, pp. 595-600, (2012) (PubMed).

Zheng, Zhang, Jiang, You, Liu, Lu, Zhou: "Functional NBS1 polymorphism is associated with occurrence and advanced disease status of nasopharyngeal carcinoma." in: Molecular carcinogenesis, Vol. 50, Issue 9, pp. 689-96, (2011) (PubMed).

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: NBN

Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Molecular Weight: 85 kDa

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response