PRKAA1 antibody

Catalog No. ABIN1731736

This Rabbit Polyclonal antibody specifically detects PRKAA1 in WB, IF, IHC (p), ICC and IP. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for PRKAA1 antibody (ABIN1731736)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PRKAA1 Antibody

Specificity: Reacts with reacts with human, mouse, rat and bovine AMP activated protein kinase, alpha-1 catalytic subunit. Based on 100 % sequence identity, this antibody is predicted to react with Chicken, Turkey, Dog, Horse, Rabbit, Guinea pig, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, West Indian ocean coelacanth, Medaka fish, Nile tilapia, Duckbill platypus and Three-spined stickleback.

Purification: Purified

Immunogen: Synthetic peptide representing a portion of the protein encoded within exon 8.

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
IHC-P0.2 - 5 μg/mL ICC/IF1 μg/mL IP2- 8 μg/mg of lysate WB 0.2 - 2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Tris citrate/ phosphate, pH 7-8

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: 4 °C

Storage Comment: This antibody is stable at 2-8°C for 1 year.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPK alpha 1

Background: 5'-AMP-activated protein kinase catalytic subunit alpha-1 (EC:2.7.11.1), AMPK subunit alpha-1 Acetyl-CoA carboxylase kinase (EC:2.7.11.27), ACACA kinase Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31), HMGCR kinase Tau-protein kinase PRKAA1 (EC:2.7.11.26) Gene name: PRKAA1, AMPK1

Gene ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect