Syntaxin 12/13 (AA 1-250) antibody

Catalog No. ABIN1742228

There are 3+ publications for this product available. The Mouse Monoclonal anti-Syntaxin 12/13 antibody is suitable to detect Syntaxin 12/13 in samples from Rat. It has been validated for ICC and WB.

Quick Overview for Syntaxin 12/13 (AA 1-250) antibody (ABIN1742228)

Target: Syntaxin 12/13

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Clone: 151-2

Product Details

Binding Specificity: AA 1-250

Purpose: Mouse anti-Syntaxin12/13 IgG

Specificity: Specific for syntaxin 12/13.

Cross-Reactivity (Details): Reacts with: rat (G3V7P1). No signal: zebrafish, mouse (Q9ER00). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 250 from rat Syntaxin12/13 (UniProt Id: G3V7P1)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:not tested yet ICC:1 : 100 up to 1 : 500, IHC:not tested yet IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Widagdo, Chai, Ridder, Chau, Johnson, Sah, Huganir, Anggono: "Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation." in: Cell reports, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Deane, Ilie, Sizdahkhani, Das Gupta, Orlowski, McKinney: "Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 595-610, (2013) (PubMed).

Target Details

Target: Syntaxin 12/13

Alternative Name: Syntaxin12/13

Background:

Synonyms: syntaxin 12, syntaxin12, syntaxin13, syntaxin 13, stx12, stx 12, stx13, stx 13, SNARES

Antigen Information: Syntaxin12/13 is a syntaxin family member functioning in the recycling of plasma membrane receptors

UniProt: G3V7P1