CACNA1E antibody (AA 1921-2222)

Catalog No. ABIN1742328

The Rabbit Polyclonal anti-CACNA1E antibody (ABIN1742328) specifically detects CACNA1E in WB. The antibody is reactive with Mouse and Rat samples.

Quick Overview for CACNA1E antibody (AA 1921-2222) (ABIN1742328)

Target: CACNA1E (Calcium Channel, Voltage-Dependent, R Type, alpha 1E Subunit (CACNA1E))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CACNA1E Antibody

Binding Specificity: AA 1921-2222

Purpose: Rabbit anti-Ca2+ channel R-type alpha-1E antibody

Specificity: Specific for Ca2+ channel alpha-1E.

Cross-Reactivity (Details): Reacts with: rat (Q07652), mouse (Q61290). Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type alpha-1E (Cav2.3) (UniProt Id: Q07652)

Application Details

Application Notes: WB:1 : 500 up to 1 : 1000 , IHC-P:not tested yet

Comment:

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Target Details for CACNA1E

Target: CACNA1E (Calcium Channel, Voltage-Dependent, R Type, alpha 1E Subunit (CACNA1E))

Alternative Name: CACNA1E

Background:

Synonyms: Cav2.3, channel alpha-1E, Cacna1e, Calcium Channel

UniProt: Q07652

Pathways: Carbohydrate Homeostasis