PAX6 antibody (AA 1-422)

Catalog No. ABIN1742329

This Mouse Monoclonal antibody specifically detects PAX6 in WB, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Chicken and has been mentioned in 2+ publications.

Quick Overview for PAX6 antibody (AA 1-422) (ABIN1742329)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Clone: AD2-38

Product Details anti-PAX6 Antibody

Binding Specificity: AA 1-422

Purpose: Mouse anti-Pax6 IgG

Specificity: Specific for Pax 6.

Cross-Reactivity (Details): Reacts with: human (P26367), rat (P63016), mouse (P63015), chicken. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 422 from mouse Pax6 (UniProt Id: P63015) Epitop AA 4 to 130 from mouse Pax6 (UniProt Id: P63015)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:not tested yet ICC:1 : 200 IHC:not tested yet IHC-P:1 : 100 up to 1 : 500,

Comment:

Nuclear extracts from tissues should be used for Western blot experiments to increase the concentration of Pax 6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-PAX6 antibody (ABIN1742329)

Vasudevan, Long, Crandall, Rubenstein, Bhide: "Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain." in: Nature neuroscience, Vol. 11, Issue 4, pp. 429-39, (2008) (PubMed).

Engelkamp, Rashbass, Seawright, van Heyningen: "Role of Pax6 in development of the cerebellar system." in: Development (Cambridge, England), Vol. 126, Issue 16, pp. 3585-96, (1999) (PubMed).

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: Pax6

Background:

Synonyms: Sey, Oculorhombin, Pax 6

Antigen Information: K.O. Pax6 is a transcription factor involved in the regulation of eye and brain development

UniProt: P63015

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis