Abeta-pE3 (AA 3-7) antibody

Catalog No. ABIN1742433

Cited in 2+ publications. The Mouse Monoclonal anti-Abeta-pE3 antibody (Clone 1-57) (ABIN1742433) specifically detects Abeta-pE3 in IHC (p) and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Abeta-pE3 (AA 3-7) antibody (ABIN1742433)

Target: Abeta-pE3

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: 1-57

Product Details

Binding Specificity: AA 3-7

Purpose: Mouse anti-Abeta-pE3 IgG

Specificity: Specific for Abeta-pE3.

Cross-Reactivity (Details): Reacts with: human (P05067), rat (P08592), mouse (P12023). Other species not tested yet.

Purification: purified IgG

Immunogen: Synthetic peptide corresponding to AA 3 to 7 from human Abeta-pE3 (UniProt Id: P05067) Epitop AA 3 to 5 from human Abeta-pE3 (UniProt Id: P05067)

Isotype: IgG2b

Application Details

Application Notes: WB:1 : 1000 ICC:not tested yet IHC:not tested yet IHC-P:1 : 1000 up to 1 : 5000,

Comment:

WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Antonios, Saiepour, Bouter, Richard, Paetau, Verkkoniemi-Ahola, Lannfelt, Ingelsson, Kovacs, Pillot, Wirths, Bayer: "N-truncated Abeta starting with position four: early intraneuronal accumulation and rescue of toxicity using NT4X-167, a novel monoclonal antibody." in: Acta neuropathologica communications, Vol. 1, Issue 1, pp. 56, (2013) (PubMed).

Wittnam, Portelius, Zetterberg, Gustavsson, Schilling, Koch, Demuth, Blennow, Wirths, Bayer: "Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8154-62, (2012) (PubMed).

Target Details

Target: Abeta-pE3

Background:

Synonyms: pE3-Abeta, pyro-Glu-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42), pyro-Abeta

Antigen Information: Abeta peptides are major components of neuritic plaques in Alzheimer's disease

UniProt: P05067