GABRA5 antibody (AA 26-43)

Catalog No. ABIN1742462

Product Details anti-GABRA5 Antibody

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Binding Specificity: AA 26-43

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for GABA-A receptor alpha5.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 26-43 of rat GABA-A receptor alpha5 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Comment:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for GABRA5

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Abstract: GABRA5 Products

Synonyms: GABRA5 antibody, si:dkey-93p24.1 antibody, A230018I05Rik antibody, gamma-aminobutyric acid type A receptor alpha5 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 5 antibody, gamma-aminobutyric acid type A receptor alpha 5 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5 antibody, gamma-aminobutyric acid receptor subunit alpha-5 antibody, GABRA5 antibody, gabra5 antibody, Gabra5 antibody, LOC100653497 antibody

Background: Synonyms: GABA(A) R alpha5

Pathways: Sensory Perception of Sound, Synaptic Membrane