GABRA5 antibody (AA 364-376)

Catalog No. ABIN7043189

The Rabbit Polyclonal anti-GABRA5 antibody is suitable to detect GABRA5 in samples from Rat. It has been validated for WB, IHC and IF.

Quick Overview for GABRA5 antibody (AA 364-376) (ABIN7043189)

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GABRA5 Antibody

Binding Specificity: AA 364-376

Purpose: A Rabbit Polyclonal Antibody to GABA(A) α5 Receptor

Specificity: 2nd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-GABA(A) alpha5 Receptor Antibody is directed against an epitope of the rat GABA(A) α5 receptor. Anti-GABA(A) α5 Receptor Antibody (ABIN7043189, ABIN7044317 and ABIN7044318) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABRA5 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IHC

Negative Control: (ABIN7235500)

Blocking Peptide: (ABIN7235500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABRA5

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Alternative Name: GABRA5

Background: γ-Aminobutyric acid receptor type A subunit α5, GABRA5,GABA(A) receptors are the main inhibitory neurotransmitter receptors in the mammalian brain. They respond to a wide variety of drugs including benzodiazepines. The entire GABA receptor family contains 19 known genes.GABA(A) receptors are mostly pentameric proteins composed of different subunits and each subunit has several subtypes. Half of each subunit consists of a hydrophilic extracellular N-terminal domain containing a Cys loop, followed by four transmembrane sequences (M1-M4). M2 lines the ion channel and between M3 and M4 there is a large intracellular loop involved in receptor modulation by phosphorylation. Five subunits form a complex and surround a central ion channel with all five M2 segments contributing to the channel1.The gene encoding the GABA(A) receptor subunit α-5 (also known as GABRA5) is mapped to chromosome 15q11-q13 (together with GABRB3 and GABRG3) and is contained within approximately 800 kb of the distal part of the imprinted Prader-Willi and Angleman syndrome region. The receptor is mostly expressed in the hippocampus and prefrontal cortex2. GABAR5 is anchored to neuronal extra synaptic regions by the cytosolic protein radixin.GABRA5 is implicated in the development of various social disorders. PET studies have shown reduced binding of a GABRA5 selective ligand in the amygdala and nucleus acumbens of people with Autism spectrum disorders (ASD). In addition, post-mortem analysis of brain tissue obtained from individuals with ASD shows reduced amounts of GABRA5 encoding DNA. Deletion of the GABRA5 gene in mice causes autism-like behavior3.

Alternative names: GABA(A) alpha5 Receptor, gamma-Aminobutyric acid receptor type A subunit alpha5, GABRA5

Gene ID: 29707

NCBI Accession: NM_000810

UniProt: P19969

Pathways: Sensory Perception of Sound, Synaptic Membrane