EEA1 antibody (AA 2-13)

Catalog No. ABIN1742484

This anti-EEA1 antibody is a Rabbit Polyclonal antibody detecting EEA1 in WB, ICC and IHC (p). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for EEA1 antibody (AA 2-13) (ABIN1742484)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EEA1 Antibody

Binding Specificity: AA 2-13

Purpose: Rabbit anti-EEA1 antiserum

Specificity: Specific for EEA 1.

Cross-Reactivity (Details): Reacts with: human (Q15075), rat (A0A0G2K051), mouse (Q8BL66). No signal: zebrafish. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 2 to 13 from human EEA1 (UniProt Id: Q15075)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:not tested yet ICC:1 : 1000 up to 1 : 2000, IHC:not recommended IHC-P:1 : 200,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-EEA1 antibody (ABIN1742484)

Pavlos, Grønborg, Riedel, Chua, Boyken, Kloepper, Urlaub, Rizzoli, Jahn: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, (2010) (PubMed).

Barysch, Aggarwal, Jahn, Rizzoli: "Sorting in early endosomes reveals connections to docking- and fusion-associated factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 24, pp. 9697-702, (2009) (PubMed).

Geumann, Barysch, Hoopmann, Jahn, Rizzoli: "SNARE function is not involved in early endosome docking." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5327-37, (2008) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background:

Synonyms: early endosomal antigen1, Early endosome antigen 1, early endosome antigen 1

Antigen Information: EEA1 is an endosomal Rab 5 effector essential for endocytic membrane fusion

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades