SLC6A1 antibody (AA 585-599)

Catalog No. ABIN1742535

This anti-SLC6A1 antibody is a Rabbit Polyclonal antibody detecting SLC6A1 in WB, IHC and ICC. Suitable for Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for SLC6A1 antibody (AA 585-599) (ABIN1742535)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SLC6A1 Antibody

Binding Specificity: AA 585-599

Specificity: Specific for GAT 1.

Purification: antiserum

Immunogen: Synthetic peptide (aa 585-599 of mouse GAT 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 500 up to 1 : 1000

Comment:

WB: GAT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SLC6A1 antibody (ABIN1742535)

Aracri, Meneghini, Coatti, Amadeo, Becchetti: "α4β2(∗) nicotinic receptors stimulate GABA release onto fast-spiking cells in layer V of mouse prefrontal (Fr2) cortex." in: Neuroscience, Vol. 340, pp. 48-61, (2016) (PubMed).

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: GABA Transporter 1

Background: Synonyms: GAT 1, Gabt1, Slc6a1