Recombinant alpha Tubulin antibody (C-Term)

Catalog No. ABIN1742547

This anti-alpha Tubulin antibody is a Mouse Monoclonal antibody detecting alpha Tubulin in WB, IHC, ELISA, IHC (p), IP and ICC. Suitable for Human, Mouse, Rat, Saccharomyces cerevisiae, Vertebrate and Invertebrate. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Recombinant alpha Tubulin antibody (C-Term) (ABIN1742547)

Target: alpha Tubulin (TUBA1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Vertebrate, Invertebrate

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 3A2

Product Details anti-alpha Tubulin Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-alpha-Tubulin IgG

Specificity: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Cross-Reactivity (Details): Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast. Other species not tested yet.

Purification: recombinant IgG

Immunogen: Synthetic peptide corresponding to residues near the carboxy terminus of human alpha-Tubulin 4A. (UniProt Id: P68366)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:yes ICC:1 : 500 up to 1 : 1000, IHC:1 : 500, IHC-P:1 : 500 up to 1 : 1000, ELISA:yes

Comment:

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-alpha Tubulin antibody (ABIN1742547)

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Background:

Synonyms: alpha-Tubulin, Tuba1a, loading control

Antigen Information: alpha-Tubulin is a major cytoskeleton protein

UniProt: P68366

Pathways: Microtubule Dynamics