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alpha Tubulin antibody (AA 443-449)

TUBA1 Reactivity: Human, Mouse, Rat, Chicken, Mammalian WB, ELISA, IHC, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1742550
  • Target See all alpha Tubulin (TUBA1) Antibodies
    alpha Tubulin (TUBA1)
    Binding Specificity
    • 12
    • 9
    • 8
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 443-449
    Reactivity
    • 98
    • 80
    • 73
    • 18
    • 17
    • 14
    • 13
    • 10
    • 9
    • 9
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Mammalian
    Host
    • 54
    • 45
    • 12
    • 2
    • 2
    • 1
    • 1
    • 1
    Rabbit
    Clonality
    • 65
    • 50
    • 2
    Polyclonal
    Conjugate
    • 75
    • 9
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Tubulin antibody is un-conjugated
    Application
    • 84
    • 37
    • 32
    • 30
    • 30
    • 25
    • 24
    • 11
    • 11
    • 10
    • 6
    • 6
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Specificity
    Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
    Purification
    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
    Immunogen
    Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
    Top Product
    Discover our top product TUBA1 Primary Antibody
  • Application Notes
    WB: 1 : 1000 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 1000
    IHC: 1 : 400
    Comment

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
    Storage
    -20 °C
    Storage Comment
    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

  • Target
    alpha Tubulin (TUBA1)
    Alternative Name
    alpha-Tubulin (TUBA1 Products)
    Pathways
    Microtubule Dynamics
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