alpha Tubulin antibody (AA 443-449)

Catalog No. ABIN1742550

Cited in 1 publication. The Rabbit Polyclonal anti-alpha Tubulin antibody (ABIN1742550) specifically detects alpha Tubulin in WB, ELISA, IHC and ICC. The antibody is reactive with Human, Mouse, Rat, Chicken and Mammalian samples.

Quick Overview for alpha Tubulin antibody (AA 443-449) (ABIN1742550)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Chicken, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 443-449

Purpose: Rabbit anti-alpha-Tubulin antibody

Specificity: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Cross-Reactivity (Details): Reacts with: human, rat (P68370-1), mouse, mammals, chicken. Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 443 to 449 from rat alpha-tubulin (UniProt Id: P68370) (UniProt Id: P68370-1)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:not recommended ICC:1 : 100 up to 1 : 1000, IHC:1 : 400, IHC-P:not tested yet ELISA:yes

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References for anti-alpha Tubulin antibody (ABIN1742550)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Background:

Synonyms: alpha-Tubulin, Tuba1a, loading control

Antigen Information: alpha-Tubulin is a major cytoskeleton protein

UniProt: P68370

Pathways: Microtubule Dynamics