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alpha Tubulin antibody (AA 443-449)

This anti-alpha Tubulin antibody is a Rabbit Polyclonal antibody detecting alpha Tubulin in WB, ELISA, IHC and ICC. Suitable for Human, Mouse, Rat, Chicken and Mammalian. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN1742550

Quick Overview for alpha Tubulin antibody (AA 443-449) (ABIN1742550)

Target

See all alpha Tubulin (TUBA1) Antibodies
alpha Tubulin (TUBA1)

Reactivity

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  • 22
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Human, Mouse, Rat, Chicken, Mammalian

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This alpha Tubulin antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 443-449

    Specificity

    Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

    Purification

    Affinity purified with the immunogen.

    Immunogen

    Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
  • Application Notes

    WB: 1:1000 up to 1:5000, IP: not recommended ICC: 1:100 up to 1:1000, IHC: 1:400,

    Comment

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

    Buffer

    PBS. Albumin was added for stabilization.

    Preservative

    Azide and BSA free

    Handling Advice

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    Storage

    -20 °C

    Storage Comment

    Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.
  • Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

  • Target

    alpha Tubulin (TUBA1)

    Alternative Name

    alpha-Tubulin

    Pathways

    Microtubule Dynamics
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