alpha Tubulin antibody (AA 443-449)

Catalog No. ABIN1742550

This anti-alpha Tubulin antibody is a Rabbit Polyclonal antibody detecting alpha Tubulin in WB, ELISA, IHC and ICC. Suitable for Human, Mouse, Rat, Chicken and Mammalian. This Primary Antibody has been cited in 1 publication.

Quick Overview for alpha Tubulin antibody (AA 443-449) (ABIN1742550)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Chicken, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 443-449

Specificity: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 400

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-alpha Tubulin antibody (ABIN1742550)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Pathways: Microtubule Dynamics