alpha Tubulin antibody (AA 443-449)

Catalog No. ABIN1742550

This anti-alpha Tubulin antibody is a Rabbit Polyclonal antibody detecting alpha Tubulin in WB, ELISA, IHC and ICC. Suitable for Human, Mouse, Rat, Chicken and Mammalian. This Primary Antibody has been cited in 1 publication.

Quick Overview for alpha Tubulin antibody (AA 443-449) (ABIN1742550)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Chicken, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 443-449

Purpose: Rabbit anti-alpha-Tubulin antibody

Specificity: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Cross-Reactivity (Details): Reacts with: human, rat (P68370-1), mouse, mammals, chicken. Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 443 to 449 from rat alpha-tubulin (UniProt Id: P68370) (UniProt Id: P68370-1)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:not recommended ICC:1 : 100 up to 1 : 1000, IHC:1 : 400, IHC-P:not tested yet ELISA:yes

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References for anti-alpha Tubulin antibody (ABIN1742550)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Background:

Synonyms: alpha-Tubulin, Tuba1a, loading control

Antigen Information: alpha-Tubulin is a major cytoskeleton protein

UniProt: P68370

Pathways: Microtubule Dynamics