LILRB1 antibody

Catalog No. ABIN1774726

This Mouse Monoclonal antibody specifically detects LILRB1 in WB, FACS, IP and Func. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for LILRB1 antibody (ABIN1774726)

Target: LILRB1 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LILRB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: GHI-75

Product Details anti-LILRB1 Antibody

Purpose: Anti-Hu CD85j Purified Low Endotoxin

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Hairy cell leukaemia cells

Isotype: IgG2b, kappa

Application Details

Application Notes: Functional application: Blocking of HLA-G induced TGF-beta1 production.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LILRB1 antibody (ABIN1774726)

Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).

Riteau, Menier, Khalil-Daher, Martinozzi, Pla, Dausset, Carosella, Rouas-Freiss: "HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition." in: International immunology, Vol. 13, Issue 2, pp. 193-201, (2001) (PubMed).

Banham, Colonna, Cella, Micklem, Pulford, Willis, Mason: "Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily." in: Journal of leukocyte biology, Vol. 65, Issue 6, pp. 841-5, (1999) (PubMed).

Target Details for LILRB1

Target: LILRB1 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))

Alternative Name: CD85j

Background: Leukocyte immunoglobulin like receptor B1,CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.,ILT2, LIR1, MIR7, PIRB, ILT-2

Gene ID: 10859

UniProt: Q8NHL6

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response