KAP1 antibody (Internal Region)

Catalog No. ABIN1781929

Product Details anti-KAP1 Antibody

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: KAP1

Sequence: KQFNKLTEDK ADVQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in nuclear lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 88.5 kDa according to NP_005753.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28 (TRIM28 Products)

Synonyms: KAP1 antibody, RNF96 antibody, TF1B antibody, TIF1B antibody, TRIM28 antibody, kap1 antibody, rnf96 antibody, tf1b antibody, tif1b antibody, AA408787 antibody, KAP-1 antibody, KRIP-1 antibody, Tif1b antibody, Tif1beta antibody, Kap1 antibody, Krip1 antibody, TIF1BETA antibody, tripartite motif containing 28 antibody, tripartite motif containing 28 L homeolog antibody, tripartite motif-containing 28 antibody, TRIM28 antibody, trim28.L antibody, Trim28 antibody

Background: TRIM28, tripartite motif-containing 28, FLJ29029, KAP1, RNF96, TF1B, TIF1B , KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 protein

Molecular Weight: Expected molecular weight: 100 kDa

Gene ID: 10155

NCBI Accession: NP_005753

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus