CD25 antibody (AA 117-129)

Catalog No. ABIN1782140

The Goat Polyclonal anti-CD25 antibody has been validated for ELISA. It is suitable to detect CD25 in samples from Human.

Quick Overview for CD25 antibody (AA 117-129) (ABIN1782140)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD25 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-CD25 Antibody

Binding Specificity: AA 117-129

Purpose: CD25 / IL2RA (aa117-129)

Sequence: DQASLPGHCR EPP

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in Human Colorectal cancer, Cerebellum, Heart and CACO-2 lysates gave no specific signal but low background (at antibody concentration up to 2 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA

Background: IL2RA, interleukin 2 receptor, alpha, CD25, IDDM10, IL2R, TCGFR, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, OTTHUMP00000019030, OTTHUMP00000019031, OTTHUMP00000019032, TAC antigen, interleukin-2 receptor subunit alpha, p55

Gene ID: 3559

NCBI Accession: NP_000408

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation