IL-11 antibody

Catalog No. ABIN181144

Product Details anti-IL-11 Antibody

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-11 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody detects Human Interleukin-11. Other species not tested.

Characteristics: Synonyms: IL-11, Adipogenesis inhibitory factor, AGIF

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human IL-11

Isotype: IgG1

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-11 (5.0 ng/mL), a concentration of 2.4-3.6 μg/mL is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL will detectat least 0.2 ng/well of recombinant Human IL-11 when used with Biotin anti-Human IL-11antibody (cat. PP1033B) as the detection antibody at a concentration of at least 2 μg/mL. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at aconcentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: Interleukin-11 / IL11 (IL11 Products)

Background: Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.Synonyms: AGIF, Adipogenesis inhibitory factor, IL-11

Gene ID: 3589

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion