Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: ELISA 1: 15,000 - 1: 60,000Western blot 1: 1,000 - 1: 6,000 Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water or equivalent.
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below.
Target
GST
(Glutathione S Transferase (GST))
Alternative Name
Glutathione-S-Transferase (GST)
Background
GST (Glutathione S-Transferase) is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.Synonyms: GST26-Tag, Glutathione S-transferase Tag