Leptin antibody

Catalog No. ABIN181608

The Goat Polyclonal anti-Leptin antibody has been validated for WB and EIA. It is suitable to detect Leptin in samples from Rat.

Quick Overview for Leptin antibody (ABIN181608)

Target: Leptin (LEP)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Leptin Antibody

Specificity: This antibody detects Leptin.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant Rat Leptin

Application Details

Application Notes: ELISA: Indirect: To detect Rat Leptin by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant Rat Leptin. Sandwich: To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-RatLeptin as a detection antibody, this anibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin. Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gene ID: 25608

NCBI Accession: NP_037208

UniProt: P50596

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process