CD22 antibody (Biotin)

Catalog No. ABIN181641

The Biotin-conjugated Rabbit Polyclonal anti-CD22 antibody (ABIN181641) specifically detects CD22 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for CD22 antibody (Biotin) (ABIN181641)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD22 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CD22 Antibody (Biotin)

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human CD22

Application Details

Application Notes: ELISA: Direct: To detect hCD22 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Sandwich: To detect hCD22 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanCD22 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCD22. Western blot: To detect hCD22 this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hCD22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22 is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140kD. It is expressed in both the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early in B cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. There are two different isoforms of CD22. It exists predominantly as a monomer of the beta isoform but can also be found as a heterodimer composed of the beta isoform and a second, shorter isoform (CD22 alpha).Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2

Gene ID: 9606

UniProt: P20273