MIA
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sectionsof human pancreas infiltrating ductal adenocarcinoma tissue. The recommendedconcentration is 1.0-2.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeledpolymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimalresults for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at roomtemperature. Optimal conditions may vary. Tissue samples were provided by theCooperative Human Tissue Network which is funded by the National Cancer Institute. Indirect ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human MIA (ABIN181670 or ABIN181669) as a Detection antibody, itallows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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Background
Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein