FGF10 antibody (Biotin)
Quick Overview for FGF10 antibody (Biotin) (ABIN181762)
Target
See all FGF10 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- This antibody detects FGF-10.
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Cross-Reactivity (Details)
- Species reactivity (tested):Human.
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Purification
- Affinity Chromatography.
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Immunogen
- E. Coli derived recombinant Human FGF-10
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Application Notes
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Direct ELISA To detect Human FGF-10 (using 100 μL/well antibody solution) a concentrationof 0.25-1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-10. Sandwich ELISA: To detect Human FGF-10 (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. In conjunction with PolyclonalAnti-Human FGF-10 (PP1119P) as a capture antibody, it allows the detection of at least0.2-0.4 ng/well of recombinant hFGF-10. Western blot: To detect Human FGF-10 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Preservative
- Without preservative
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Handling Advice
- Centrifuge vial prior to opening!
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Storage
- 4 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should protected from light.
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- FGF10 (Fibroblast Growth Factor 10 (FGF10))
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Alternative Name
- FGF10
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Background
- FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.Synonyms: FGF-10, Fibroblast growth factor 10, KGF2, Keratinocyte growth factor 2
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Gene ID
- 2255
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NCBI Accession
- NP_004456
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UniProt
- O15520
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus
Target
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