IL-2 antibody

Catalog No. ABIN181831

This Mouse Monoclonal antibody specifically detects IL-2 in EIA and Func. It exhibits reactivity toward Chicken, Avian and Mammalian.

Quick Overview for IL-2 antibody (ABIN181831)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Chicken, Avian, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 4F12

Product Details anti-IL-2 Antibody

Specificity: This antibody SM1540A recognises natural and recombinant IL-2 expressed in either E.coli or CHO cells. Clone 4F12 neutralises the proliferative activity of rchIL-2 and recognises a different epitope to Clone 10E7 product code (Cat.-No SM1539A). This antibody can be used as the Neutralising antibody for recombinant Chicken IL-2 bioassays (See Product code SA028).

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant Chicken IL-2. Remarks: Spleen cells from immunised mice were fused with cells of the mouse Sp2/0-Ag myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: ELISA: 5-10g as capture antibody. Western Blot: Non-reducing conditions. Functional Assays: 2-20g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives, None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 / IL2

Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF

Gene ID: 3558

NCBI Accession: NP_000577

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation