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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This His Tag antibody is conjugated to FITC
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS)
- Sequence
- HHHHHH
- Purification
- Affinity Chromatography on Protein G
- Immunogen
- PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Clone
- AD1-1-10
- Isotype
- IgG1
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA Host: Rabbit Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP Host: Mouse Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyReactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated
anti-His Tag (C-Term) antibodyWB, ELISA Host: Rabbit Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt Host: Mouse Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (DyLight 649)WB, ELISA, DB, FM, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (DyLight 800)WB, ELISA, DB, FM, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 800
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- Application Notes
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Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- His Tag
- Alternative Name
- 6xHistidine Epitope Tag (His Tag Products)
- Target Type
- Tag
- Background
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
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