GM-CSF antibody

Catalog No. ABIN181873

This Mouse Monoclonal antibody specifically detects GM-CSF in WB and EIA. It exhibits reactivity toward Sheep.

Quick Overview for GM-CSF antibody (ABIN181873)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 3C2

Product Details anti-GM-CSF Antibody

Specificity: This antibody recognises recombinant and native GM-CSF. Clone 8D8 has been reported to neutralise the activity of both recombinant and native GM-CSF.

Purification: Affinity chromatography on Protein G

Immunogen: CHO expressed recombinant ovine GM-CSF. Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Western Blot. ELISA: 1/100 as detecting antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 443400

NCBI Accession: NP_001009805

UniProt: P28773

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin