SIRPA antibody
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- Target See all SIRPA Antibodies
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
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Reactivity
- Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SIRPA antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Porcine CD172a, a member of the signal regulatory protein (SIRP) family. Clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (,). CD172a is expressed on monocyte derived dendritic cells (MoDCs) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(, ). Clone BL1H7 immunoprecipitated a single band of ~90-110kD from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions. Has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues. Aberrant expression of CD172a has been noted on porcine leukemias with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a as opposed to the more characteristic of CD172+ cells seen in normal blood PBMC.
- Purification
- Protein G purified
- Immunogen
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Porcine alveolar macrophages.
Type of Immunogen: Cells - Clone
- BL1H7
- Isotype
- IgG1
- Top Product
- Discover our top product SIRPA Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Target Species of Antibody: Pig
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Store undiluted.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.
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- Target
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- Alternative Name
- CD172A / SIRPA (SIRPA Products)
- Synonyms
- BIT antibody, CD172A antibody, MFR antibody, MYD-1 antibody, P84 antibody, PTPNS1 antibody, SHPS1 antibody, SIRP antibody, SIRPA antibody, SIRP-A1 antibody, SIRP-B1 antibody, AI835480 antibody, Bit antibody, CD172a antibody, Ptpns1 antibody, SHP-1 antibody, SHPS-1 antibody, swc3 antibody, signal regulatory protein alpha antibody, myeloperoxidase antibody, signal-regulatory protein alpha antibody, tyrosine-protein phosphatase non-receptor type substrate 1 antibody, SIRPA antibody, MPO antibody, LOC458039 antibody, LOC100400378 antibody, LOC100590224 antibody, Sirpa antibody
- Background
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Name/Gene ID: SIRPA
Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2 - Gene ID
- 140885
- UniProt
- P78324
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