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CD14 antibody

CD14 Reactivity: Human, Cow, Sheep, Water Buffalo FACS, IF Host: Mouse Monoclonal CC-G33 unconjugated

Catalog No. ABIN1819121

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Reactivity
226

40

37

25

24

21

21

21

17

16

15

14

13

13

12

9

8

7

5

5

5

2

1

1
Human, Cow, Sheep, Water Buffalo
Host
198

54

16

3

1
Mouse
Compatible Secondaries
Clonality
216

55

1
Monoclonal
Conjugate
119

30

22

18

12

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
202

86

63

51

48

46

20

15

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognizes an epitope on ovine CD14, see for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo.

Purification

Protein G purified

Immunogen

Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Type of Immunogen: Purified protein

Clone

CC-G33

Isotype

IgG1

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CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

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anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Target Species of Antibody: Bovine

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C,-20 °C

Storage Comment

4°C or -20°C, Avoid freeze-thaw cycles.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Background

Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades