CD14 antibody

Catalog No. ABIN1819121

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS and IF. It is suitable to detect CD14 in samples from Human, Cow, Sheep and Water Buffalo.

Quick Overview for CD14 antibody (ABIN1819121)

Target: CD14

Reactivity: Human, Cow, Sheep, Water Buffalo

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CC-G33

Product Details anti-CD14 Antibody

Specificity: Recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognizes an epitope on ovine CD14, see for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo.

Purification: Protein G purified

Immunogen: Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades