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His Tag antibody (Biotin)

This anti-His Tag antibody is a Goat Polyclonal antibody detecting His Tag in WB and ELISA. Suitable for .
Catalog No. ABIN1819362

Quick Overview for His Tag antibody (Biotin) (ABIN1819362)

Target

See all His Tag Antibodies
His Tag

Reactivity

Please inquire

Host

  • 107
  • 65
  • 10
  • 10
  • 9
  • 8
  • 2
  • 1
Goat

Clonality

  • 122
  • 75
  • 11
Polyclonal

Conjugate

  • 94
  • 20
  • 13
  • 13
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This His Tag antibody is conjugated to Biotin

Application

  • 167
  • 115
  • 40
  • 33
  • 31
  • 30
  • 28
  • 16
  • 15
  • 13
  • 13
  • 13
  • 7
  • 6
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Sequence

    HHHHHH

    Specificity

    Recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. May not detect all N-Terminal (NT) histidine tagged proteins. Has been shown to be suitable for use on cytospins.

    Purification

    Affinity purified

    Immunogen

    Keyhole limpet hemocyanin (KLH) conjugated H-H-H-H-H-H.

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (1:10000 - 1:100000), WB (1:10000 - 1:100000)

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 0.09 % sodium azide, 0.2 % BSA

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C

    Storage Comment

    Store at 4°C. Do not freeze.
  • Target

    His Tag

    Target Type

    Tag
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