CD4 antibody (FITC)

Catalog No. ABIN181937

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Chicken.

Quick Overview for CD4 antibody (FITC) (ABIN181937)

Target: CD4

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 01-02-35

Product Details anti-CD4 Antibody (FITC)

Purification: Affinity chromatography on Protein G

Immunogen: Chicken embryonic thymocytes. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 9031

UniProt: Q6Q4G0

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events