PRKAA1 antibody (N-Term)

Catalog No. ABIN1819880

The Mouse Monoclonal anti-PRKAA1 antibody is suitable to detect PRKAA1 in samples from Human, Mouse, Rat, Drosophila melanogaster and Vertebrate. It has been validated for WB, IHC, ELISA, IF, IP and IHC (fro).

Quick Overview for PRKAA1 antibody (N-Term) (ABIN1819880)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Drosophila melanogaster, Vertebrate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 34-2

Product Details anti-PRKAA1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilized by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels. AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.

Purification: Protein G purified

Immunogen: 29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IP, WB (1:100 - 1:1000)

Comment:

Target Species of Antibody: Drosophila

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1 / AMPK Alpha 1

Background: Name/Gene ID: PRKAA1
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase

Gene ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect