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CD14 antibody (FITC)

CD14 Reactivity: Human, Cow, Sheep, Water Buffalo FACS, IF Host: Mouse Monoclonal CC-G33 FITC

Catalog No. ABIN1819883

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Reactivity
230

41

37

25

24

21

21

21

17

16

15

14

13

13

12

9

8

7

5

5

5

2

1

1
Human, Cow, Sheep, Water Buffalo
Host
199

56

16

3

2

1
Mouse
Compatible Secondaries
Clonality
219

57

1
Monoclonal
Conjugate
127

29

21

18

12

5

4

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is conjugated to FITC
Application
204

91

68

56

48

44

20

15

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Recognizes bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognizes an epitope on ovine CD14.

Purification

Protein G purified

Immunogen

Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Type of Immunogen: Purified protein

Clone

CC-G33

Isotype

IgG1

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CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

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anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo, IF

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Bovine

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. Protect from light.

Storage

4 °C,-20 °C

Storage Comment

Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Background

Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID

929

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades