CD4 antibody (PE)

Catalog No. ABIN182021

The Rat Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Dog.

Quick Overview for CD4 antibody (PE) (ABIN182021)

Target: CD4

Reactivity: Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: YKIX302-9

Product Details anti-CD4 Antibody (PE)

Specificity: This antibody recognises the CD4 cell surface antigen. The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro.

Purification: Affinity Chromatography on Protein G

Immunogen: Canine Concanavilin A activated T-cell blasts. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.Reconstitution: Restore with 1.0 ml distilled water.

Expiry Date: 12 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: In the dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 403931

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events