alpha Tubulin antibody (Subunit alpha) (HRP)

Catalog No. ABIN1820258

The Rat Monoclonal anti-alpha Tubulin antibody has been validated for WB, ELISA, IHC and IHC (fro). It is suitable to detect alpha Tubulin in samples from Human, Mouse, Rat, Saccharomyces cerevisiae, Arabidopsis, Drosophila melanogaster, Xenopus laevis and C. elegans.

Quick Overview for alpha Tubulin antibody (Subunit alpha) (HRP) (ABIN1820258)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Arabidopsis, Drosophila melanogaster, Xenopus laevis, C. elegans

Host: Rat

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YL1-2

Product Details anti-alpha Tubulin Antibody (HRP)

Binding Specificity: Subunit alpha

Specificity: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

No Cross-Reactivity: Nephrotoma

Purification: Protein G purified

Immunogen: Yeast tubulin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB (1:100 - 1:1000)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % Thimerosal, HRP stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Pathways: Microtubule Dynamics