IL-8 antibody

Catalog No. ABIN182115

The Rabbit Polyclonal anti-IL-8 antibody (ABIN182115) specifically detects IL-8 in EIA. The antibody is reactive with Sheep samples.

Quick Overview for IL-8 antibody (ABIN182115)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-IL-8 Antibody

Purification: None

Immunogen: Recombinant ovine IL-8

Application Details

Application Notes: ELISA (1/500 for detection, Use ABIN120891 as coating antibody, It is recommended that ABIN182115 and any detection antibody are diluted in 10 % normal mouse serum prior to use in ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Serum stabilized by 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C or at -20 °C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: One year from despatch.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8

Background: Interleukin 8, IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy