Histone H2B antibody (ubLys120)

Catalog No. ABIN1822545

This anti-Histone H2B antibody is a Rabbit Monoclonal antibody detecting Histone H2B in WB. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Histone H2B antibody (ubLys120) (ABIN1822545)

Target: Histone H2B

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB)

Clone: B1249M

Product Details anti-Histone H2B Antibody

Binding Specificity: ubLys120

Specificity: Endogenous levels of histone H2B, ubiquitylated on Lys120. Species Crossreactivity: monkey, mouse, rat.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the carboxy terminus of the human histone H2B protein in which Lys120 is mono-ubiquitylated.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Histone H2B

Target: Histone H2B