Histone H2B antibody (acLys12)

Catalog No. ABIN1822547

The Rabbit Polyclonal anti-Histone H2B antibody has been validated for WB, IF and IP. It is suitable to detect Histone H2B in samples from Human, Mouse, Rat and Monkey.

Quick Overview for Histone H2B antibody (acLys12) (ABIN1822547)

Target: Histone H2B

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Histone H2B Antibody

Binding Specificity: acLys12

Specificity: Endogenous levels of histone H2B, acetylated at Lys12. Species Crossreactivity: monkey, mouse, rat.

Purification: Affinity purified

Immunogen: Synthetic peptide containing acetylated Lys12 of human histone H2B.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100), IP (1:100), WB

Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Histone H2B

Target: Histone H2B