IFIH1 antibody (Arg470)

Catalog No. ABIN1822663

This Rabbit Monoclonal antibody specifically detects IFIH1 in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for IFIH1 antibody (Arg470) (ABIN1822663)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IFIH1 Antibody

Binding Specificity: Arg470

Specificity: Endogenous levels of total MDA-5. Species Crossreactivity: mouse.

Purification: Purified

Immunogen: Synthetic peptide corresponding to residues surrounding Arg470 of human MDA-5.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:100), WB

Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: IFIH1 / MDA5

Background: Name/Gene ID: IFIH1
Family: DEAD box protein

Synonyms: IFIH1, Helicase with 2 CARD domains, MDA-5, MDA5, Helicard, IDDM19, RLR-2, RH116, CADM-140 autoantigen, Hlcd, RIG-I-like receptor 2

Gene ID: 64135

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response