Afadin antibody (pSer1718)

Catalog No. ABIN1831232

The Rabbit Polyclonal anti-Afadin antibody has been validated for WB. It is suitable to detect Afadin in samples from Human, Rat, Mouse, Dog and Monkey.

Quick Overview for Afadin antibody (pSer1718) (ABIN1831232)

Target: Afadin (MLLT4)

Reactivity: Human, Rat, Mouse, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Afadin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Afadin Antibody

Binding Specificity: pSer1718

Specificity: Endogenous levels of l-afadin protein only when phosphorylated at serine 1718.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: Western Blot: Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Afadin

Target: Afadin (MLLT4)

Alternative Name: MLLT4 / Afadin

Background: Name/Gene ID: MLLT4

Synonyms: MLLT4, Afadin, AF6, AF-6, Translocated to, 4, Protein AF-6, RP3-431P23.3

Gene ID: 4301

UniProt: P55196

Pathways: Cell-Cell Junction Organization