CD19 antibody (C-Term)

Catalog No. ABIN1838100

The Mouse Monoclonal anti-CD19 antibody (Clone LE-CD19) (ABIN1838100) specifically detects CD19 in FACS, WB, IHC, IP, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD19 antibody (C-Term) (ABIN1838100)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LE-CD19

Product Details anti-CD19 Antibody

Binding Specificity: C-Term

Specificity: LE-CD19 recognizes CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells.

Purification: Purified

Immunogen: Synthetic peptide derived from the c-terminus of human CD19.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-P (1:100 - 1:200), IP, WB

Usage: Immunohistochemistry: Suggested dilution: 1/100 - 1/200. Antigen retrieval using heat treatment methods prior to staining of paraffin sections isrecommended(Sodium citrate  pH 6.0). Positive control: tonsil. Flow cytometry: Use 10 μL to label 1x106 cells in 100 μL. Membrane permeabilisation is required for this application. Western Blot: Detects a band of approximately 95  kDa in Raji cell lysates under reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: Name/Gene ID: CD19

Synonyms: CD19, B-lymphocyte antigen CD19, CD19 Molecule, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CD19 antigen, CVID3

Gene ID: 930

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway