GST antibody

Catalog No. ABIN7477938

This Rabbit Polyclonal antibody specifically detects GST in WB and ELISA. It exhibits reactivity toward Schistosoma japonicumand has been mentioned in 1 publication.

Quick Overview for GST antibody (ABIN7477938)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GST Antibody

Specificity: This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.

Characteristics: Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant Glutathione-S-Transferase from Schistosoma japonicum

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 5 µg/mL
Western blot: 0. 1-1 µg/mL
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

References for anti-GST antibody (ABIN7477938)

Bidlingmaier, Wang, Liu, Zhang, Liu: "Comprehensive analysis of yeast surface displayed cDNA library selection outputs by exon microarray to identify novel protein-ligand interactions." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 3, pp. M110.005116, (2011) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)