ITK antibody (C-Term)

Catalog No. ABIN184605

This anti-ITK antibody is a Goat Polyclonal antibody detecting ITK in WB, ELISA and IF. Suitable for Human.

Quick Overview for ITK antibody (C-Term) (ABIN184605)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ITK Antibody

Binding Specificity: C-Term

Purpose: ITK

Sequence: RLLRQLAEIA ESGL

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentrat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background: ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258

Gene ID: 3702, 16428

NCBI Accession: NP_005537

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway