CSK antibody (C-Term)

Catalog No. ABIN184606

This Goat Polyclonal antibody specifically detects CSK in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CSK antibody (C-Term) (ABIN184606)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CSK Antibody

Binding Specificity: C-Term

Purpose: CSK

Sequence: EQLEHIKTHE LHL

Specificity: Reported variants represent identical protein: NP_004374.1, NP_001120662.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQLEHIKTHELHL, from the C Terminus of the protein sequence according to NP_004374.1, NP_001120662.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of Jurkat, K562 and MOLT4 (calculated MW of 50.7 kDa according to NP_004374.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: CSK

Background: CSK, c-src tyrosine kinase, MGC117393

Gene ID: 1445, 12988, 315707

NCBI Accession: NP_004374

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events