SMAD4 antibody (C-Term)

Catalog No. ABIN184619

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Purpose: SMAD4 / MADH4

Sequence: HTMPIADPQP LD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HTMPIADPQPLD, from the C Terminus of the protein sequence according to NP_005350.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave a band at approx 75 kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Background: MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, SMAD family member 4, OTTHUMP00000163548, SMAD,

Gene ID: 4089, 50554

NCBI Accession: NP_005350

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy